The Zymo-Spin™ IIC Fast-Spin column can be used either in microcentrifuges or on vacuum manifolds for the purification of DNA and/or RNA. The Zymo-Spin™ IIC features durable polypropylene construction and contains a unique silica-based matrix that allows purification of up to 25 µg DNA or RNA in ≥ 25 µl eluate. Capacity is 900 µl.

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below). The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ IIC Column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.).

The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI Reagent® or similar. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.). Isolation of RNA by conventional phase separation was shown to selectively enrich for some species of miRNA, leading to bias in downstream analysis. The Direct-zol™ method assures unbiased recovery of small RNAs including miRNA (see below). The procedure is easy. Simply apply a prepared sample in TRI Reagent® directly to the Zymo-Spin™ IIC Column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The eluted RNA is high quality and suitable for subsequent molecular manipulation and analysis (including RT-PCR, transcription profiling, hybridization, sequencing etc.). The entire procedure typically takes only 10 minutes.

Format Spin Column DNA Recovery Typically, up to 25 µg total DNA can be eluted into as little as 25 µl water. For DNA 50 bp to 10 kb the recovery is 70-90%. For DNA 11 kb to 23 kb the recovery is 50-70%. Equipment Microcentrifuge DNA Size Limits 50 bp to 23 kb Sample Sources DNA from PCR, restriction endonuclease digestions, plasmid preparations, kinase reactions, etc. DNA Purity High-quality, purified DNA is eluted with water and is especially well suited for sequencing, ligation reactions, and restriction endonuclease digestions. Product Detergent Tolerance ≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤0.1% SDS